ABSTRACT
Background: The likelihood of developing end-stage renal disease in an individual is determined by interactions between genetic and environmental factors. Human leukocyte antigen is the most polymorphic genetic system in man. Genes of this region influence susceptibility to certain diseases
Objective: The purpose of the present study is to investigate the association of HLA class I [HLA-A and HLA-B] with the end-stage renal disease in Iraqi patients [Arab and Kurd]
Subjects and Methods: HLA-typing was assessed in 200 patients with end-stage renal disease and 110 healthy controls by microlymphocytotxicity assay
Results: A survey of the distribution of HLA-A and HLA-B antigens frequencies yielded a significant variation between patients and healthy control group. Arab patients have significant increase in frequency of HLA-A2 as compared with healthy control [P=0.005]. Whereas Kurdish patients revealed significant increase in frequency of HLA-B35 when compared with healthy control [P=0.033]
Conclusion: The current study suggests that high frequency of HLA-A2 in Arab patients and HLA-B35 in Kurdish patients might be associated with susceptibility to risk of end-stage renal disease
ABSTRACT
The separation and determination of some anticonvnlsant drugs were done in some pharmaceutical preparations and in serum by high-performance liquid chromatography reverse phase. The chromatographic condition were: mobile phase Acetonitril - Methanol - 0.06 M Potassium phosphate 25: 15: 60 W/W pH 4.0, column of octadecyle 5 mico [250 mm x 46 mm] Nucleosil with guard column. The extraction method was based on pharmacopia procedures for pharmaceutical preparations, and the technique used solid / liquid [SEP] for serum. This method permits the Simultaneous determination of all these compounds in pharmaceutical forms for different companies and in serum. Our method is simple and sensitive, based on qualitative and quantitative studies, the Coefficient regression was 0.9998 and Coefficient of variation was between 0.46% - l.t7% and the limit of detection was between 0.9%. Consequently our results showed a very good correlation and they were useful for quality control in the drug industries, the poisoning control centers and in medical laboratories